Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3

Synonyms CINC-2beta, MIP2-beta

Accession Q10746

GeneID 171551

Source Escherichia coli.

Molecular Weight Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSPSL

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin Less than 1 EU/μg of rRtCINC-2β/CXCL3 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.

2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.

3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.

5. Nakagawa H, Komorita N, Shibata F, et al. 1994. Biochem J. 301 ( Pt 2):545-50.

Background CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GROγ in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3α/CINC-2α and CXCL3β/CINC-2β. CXCL3α differs from CXCL3β as DKSS to PSL (98-101) at carboxy-terminal residues.