Recombinant Exendin-4

Synonyms Exenatide

Accession P26349

Source Escherichia coli.

Molecular Weight Approximately 4.2 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids.

Quantity 20µg/100µg/1mg

AA Sequence HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 1. Regulates Glucose levels rapidly;

2. Reduces Insulin resistance;

3. Reduces Glucagon;

4. Reduces HbA1c;

5. Stimulates beta cell growth which stimulates insulin production.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.

Endotoxin Less than 10 EU/mg of rExendin-4 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Malhotra R, Singh L, Eng J, et al. 1992. Regul Pept, 41: 149-56.

2. Neidigh JW, Fesinmeyer RM, Prickett KS, et al. 2001. Biochemistry, 40: 13188-200.

3. Movassat J, Beattie GM, Lopez AD, et al. 2002. J Clin Endocrinol Metab, 87: 4775-81.

4. Alarcon C, Wicksteed B, Rhodes CJ. 2006. Diabetologia, 49: 2920-9.

Background Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes ß-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4.