Accession P06179

GeneID 1253480

Source Escherichia coli.

Molecular Weight Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.

Quantity 10µg/50µg/1mg

AA Sequence MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Data is not available.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rFlagellin, His as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8.

2. Belas RandFlaherty D. 1994. Gene, 148: 33-41.

3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53.

4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94.

5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.

Background Flagellin arranges itself in a hollow cylinder to form the filament in bacterial flagellum. It is the principal substituent of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium and the plant defense mechanisms can be activated by the conserved N-terminal part of flagellin.