Recombinant Rat Interleukin-6

Accession P20607

GeneID 24498

Source Escherichia coli.

Molecular Weight Approximately 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids

Quantity 2µg/10µg/1mg

AA Sequence FPTSQVRRGD FTEDTTHNRP VYTTSQVGGL ITYVLREILE MRKELCNGNS DCMNSDDALS ENNLKLPEIQ RNDGCFQTGY NQEICLLKIC SGLLEFRFYL EFVKNNLQDN KKDKARVIQS NTETLVHIFK QEIKDSYKIV LPTPTSNALL MEKLESQKEW LRTKTIQLIL KALEEFLKVT MRSTRQT

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtIL-6 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.

2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.

3. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.

4. Northemann W, Braciak TA, Hattori M, et al. 1989. J Biol Chem. 264:16072-82.

5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.

6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.

Background Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. IL-16 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The rat IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal-transducing component gp130 (also called CD130). The rat IL-6 shares 40 % and 85 % aa sequence identity with human and mouse IL-6 and it is equally active on human and mouse cells.