Recombinant Murine Interleukin-1 alpha

Synonyms Lymphocyte-activating factor, LAF, Leukocyte Endogenous Mediator, LEM, Endogenous Pyrogen, EP, Mononuclear Cell Factor, MCF, Hematopoietin-1

Accession Q3U0Y6

GeneID 16175

Source Escherichia coli.

Molecular Weight Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 108 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuIL-1α as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

Background Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. Among various species,the amino acid sequence of mature IL-1α s conserved 60 % to 70 % and human IL1 has been found to be biologically active on murine cell lines .