Recombinant Human Fibroblast Growth Factor 13

Synonyms FHF-2

Accession Q92913

GeneID 2258

Source Escherichia coli.

Molecular Weight Approximately 27.6 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence MAAAIASSLI RQKRQARERE KSNACKCVSS PSKGKTSCDK NKLNVFSRVK LFGSKKRRRR RPEPQLKGIV TKLYSRQGYH LQLQADGTID GTKDEDSTYT LFNLIPVGLR VVAIQGVQTK LYLAMNSEGY LYTSELFTPE CKFKESVFEN YYVTYSSMIY RQQQSGRGWY LGLNKEGEIM KGNHVKKNKP AAHFLPKPLK VAMYKEPSLH DLTEFSRSGS GTPTKSRSVS GVLNGGKSMS HNEST

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Test in process.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.5, 500 mM NaCl.

Endotoxin Less than 1 EU/µg of rHuFGF-13 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.

2. Wittmack EK, Rush AM, Craner MJ, et al. 2004. J Neurosci. 24:6765-75.

3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.

4. Olsen SK, Garbi M, Zampieri N, et al. 2003. J Biol Chem. 278:34226-36.

5. Hartung H, Feldman B, Lovec H, et al. 1997. Mech Dev. 64:31-9.

Background Human FGF-13 encoded by the FGF13 gene, belongs to the FGF-11 subfamily which has four members FGF-11 to FGF-14. These four members were initially referred to as fibroblast growth factor homologous factors (FHFs) because they have high sequence identity with the other FGFs but did not activate FGF receptors (FGFRs) and were not generally considered members of the FGF family. FGF-13 plays a crucial role in neuron polarization and migration in the cerebral cortex. In murine FGF-13 RNA was detected in developing central nervous system in cells, and was also found throughout the peripheral nervous system.