YBB101Mu01 100µg

Recombinant Nucleoporin 50kDa (NUP50)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]
                                                                                   

Residues: Asn134~Val393 (Accession # Q9JIH2), with two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Nucleus, nuclear pore

complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 31.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NNSNQPP SSGPASSTAC

PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL ENGGGSSSES

QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV EFTAEKKSDA AQGATSASFS

FGKKIESSAL GSLSSGSLTG FSFSAGSSSL FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE

CRDGEEEEND EPPKVVVTEV KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ

LLV

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