YBA029Ca01

Polyclonal Antibody to Selectin, Endothelium (SELE)

Organism Species: Canis familiaris; Canine (Dog)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: SELE, Canine Purification: Affinity Chromatography.

Clonality: Polyclonal Applications: WB, ICC, IHC-P, IHC-F, ELISA

Host: Rabbit Concentration: 200µg/mL

Immunoglobulin Type: IgG UOM: 100µg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant SELE (Trp23~Val240) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli.

Accession No.: RPA029Ca01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against SELE. It has been selected for its ability to recognize SELE in immunohistochemical staining and western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-5000

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant SELE (Trp23~Val240) disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per we ll wh en used in enhan ced ch emil umes cen t (E C L) .

Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]

Used in Western Blot, Sample

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