YB91697Mu01

Alpha-1-antitrypsin 1-1(Serpina1a)

Organism: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Alpha-1-antitrypsin 1-1

Gene Names: Serpina1a

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

Subcellular Location: Secreted

[ PROPERTIES ]

Residues: Glu25~Lys413 (Accession # Q7Z4T9), with a N-terminal His-tag.

Grade & Purity: >97%, 45.03 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 45.03 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-EDVQET  DTSQKDQSPA SHEIATNLGD  FAISLYRELV HQSNTSNIFF  SPVSIATAFA

MLSLGSKGDT  HTQILEGLQF  NLTQTSEADI  HKSFQHLLQT  LNRPDSELQL STGNGLFVNN  DLKLVEKFLE

EAKNHYQAEV FSVNFAESEE AKKVINDFVE KGTQGKIAEA VKKLDQDTVF ALANYILFKG KWKKPFDPEN

TEEAEFHVDE STTVKVPMMT LSGMLHVHHC STLSSWVLLM DYAGNATAVF LLPDDGKMQH LEQTLSKELI

SKFLLNRRRR LAQIHFPRLS ISGEYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSQ AVHKAVLTID

ETGTEAAAVT VLQMVPMSMP PILRFDHPFL FIIFEEHTQS PIFLGKVVDP THK

[ REFERENCES ]

1.Paterson T., Moore S. (1996) Biochem. Biophys. Res. Commun. 219:64-69.

2.Barbour K.W., et al. (2002) Mol. Biol. Evol. 19:718-727.

3.Barbour K.W., et al. (2002) Genomics 80:515-522.

4.The MGC Project Team.(2004) Genome Res. 14:2121-2127.

5.Krauter K.S., et al. (1986) DNA 5:29-36.

6.Bernhard O.K., Kapp E.A., Simpson R.J. (2007) J. Proteome Res. 6:987-995.

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