YB90112Mu01

Betacellulin (bTC)

Organism: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

3th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Betacellulin

Gene Names: bTC

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Subcellular Location: Secreted, extracellular space.

Tissue Specificity: Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.

[ PROPERTIES ]

Residues: Asp32~Gln118 (Accession # Q05928), with a N-terminal His-tag.

Grade & Purity: >97%, 11.4 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 11.4 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSE F-DGNTTRTPE TNGSLCGAP G ENCTGT TPRQ KVKTHF SRCP KQYKHYCIHG

RCRFVVDEQT PSCICEKGYF GARCERVDLF YLQQDRGQ

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