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半乳糖苷酶β(GLβ)重组蛋白
半乳糖苷酶β(GLβ)重组蛋白

半乳糖苷酶β(GLβ)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and p

Product Introduction


YBA196Hu01 100µg


Galactosidase Beta (GLb)


Organism Species: Homo sapiens (Human)


Instruction manual


FOR IN VITRO USE AND RESEARCH USE ONLY


NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES



9th Edition (Revised in Jul, 2013)


[ PROPERTIES ]
                     

Residues: Arg68~Thr219 (Accession # P16278), with two N-terminal Tags, His-tag and S-tag.


Host: E. coli


Subcellular Location: Lysosome. Cytoplasm,


perinuclear region.


Purity: >95%


Endotoxin Level: <1.0EU per 1μg


(determined by the LAL method).


Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.6


Predicted Molecular Mass: 23.4kDa


Applications: SDS-PAGE; WB; ELISA; IP.


(May be suitable for use in other assays to be determined by the end user.)


[ USAGE ]



Reconstitute in sterile PBS, pH7.2-pH7.4.




[ STORAGE AND STABILITY ]


Storage: Avoid repeated freeze/thaw cycles.


Store at 2-8oC for one month.


Aliquot and store at -80oC for 12 months.


Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.


[ SEQUENCES ]


The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- RLL


KMKMAGLNAI QTYVPWNFHE PWPGQYQFSE DHDVEYFLRL AHELGLLVIL RPGPYICAEW


EMGGLPAWLL EKESILLRSS DPDYLAAVDK WLGVLLPKMK PLLYQNGGPV ITVQVENEYG


SYFACDFDYL RFLQKRFRHH LGDDVVLFT

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