YB86792Ra01

Galectin 5 (GAL5)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

3th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Galectin 5

Gene Names: GAL5

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: May function in erythrocyte differentiation.

Tissue Specificity: Erythrocytes.

[ PROPERTIES ]

Residues: Met1~Thr145 (Accession # P47967), with a N-terminal His-tag.

Grade & Purity: >97%, 17.4 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 17.4 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-MSSFSTQTPY PNLAVPFFTS IPNGLYPSKS IVISGVVLSD AKRFQINLRC GGDIAFHLNP

RFDENAVVRN TQINNSWGPE ERSLPGSMPF SRGQRFSVWI LCEGHCFKVA VDGQHICEYS HRLMNLPDIN

TLEVAGDIQL THVET

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