YB91756Hu01

Transglutaminase 3, Epidermal (TGM3)

Organism: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION ]

Protein Names: Transglutaminase 3, Epidermal Human TGM3

Synonyms: TGM3

Species: Human

Size: 10µg

Source: Escherichia coli-derived

[ PROPERTIES ]

Residues: Ala468~Glu693 (Accession # Q08188), with N-terminal His-Tag.

Grade & Purity: >95%, 28kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 26.3kDa

Predicted isoelectric point: 5.5

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGS- ATS SMGLETEEQE PSIIGKLKVA GMLAVGKEVN LVLLLKNLSR

DTKTVTVNMT AWTIIYNGTL VHEVWKDSAT MSLDPEEEAE HPIKISYAQY EKYLKSDNMI

RITAVCKVPD ESEVVVERDI ILDNPTLTLE VLNEARVRKP VNVQMLFSNP LDEPVRDCVL

MVEGSGLLLG NLKIDVPTLG PKEGSRVRFD ILPSRSGTKQ LLADFSCNKF PAIKAMLSID

VAE

[ REFERENCES ]

1.Liu J., et al. (2012) Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 26:101-103.

2.Zone J.J., et al. (2011) J. Immunol. 186:4474-4480.

3.Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.

4.Yamane A., et al. (2010) FEBS J. 277:3564-3574.

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