YB92646Hu22

Monoclonal Antibody to Myotubularin 1 (MTM1)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in September, 2012)

[ PRODUCT INFORMATION ]

Immunogen: MTM1

Clonality: Monoclonal

Host: Mouse

Species Reactivity: Human

Ig type: Mouse IgG

Purification: Antibodies are purified by target protein affinity chromatography.

Applications: WB, ICC, IHC-P, IHC-F

Form: Liquid

Size: 100ug

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant human MTM1 (His315~Cys482) expressed in E.coli.

Molecular Weight: 20.7 kDa

USCN accession No.: P92646Hu01

Sequence: The target protein is fused with a His-tag and its sequence is listed below.

MGHHHHHHSGS-HVMRES LKKVKDIVYP NVEESHWLSS LESTHWLEHI KLVLTGAIQV ADKVSSGKSS VLVHCSDGWD

RTAQLTSLAM  LMLDSFYRSI  EGFEILVQKE  WISFGHKFAS RIGHGDKNHT  DADRSPIFLQ  FIDCVWQMSK  QFPTAFEFNE

QFLIIILDHL YSCRFGTFLF NC

[ ANTIBODY SPEFICITY ]

Anti MTM1 is a mouse monoclonal antibody raised against human MTM1. This antibody has been selected for its ability to recognize human MTM1 in immunohistochemiscal staining and western blotting, non cross-reactive with other members of the family.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N, 50% glycerol.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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