YB90041Ra01

Neutrophil Activating Protein 3 (NAP3)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Neutrophil Activating Protein 3; C-X-C motif chemokine 1;

Growth-regulated alpha protein

Gene Names: Cxcl1, Cinc1, Gro, Scyb1

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.

Subcellular Location: Secreted

Tissue Specificity: At least expressed in the lung and trachea.

[ PROPERTIES ]

Residues: Ala25~Lys96 (Accession # P14095), with a N-terminal His-tag.

Grade & Purity: >97%, 11.67 kDa as determined by SDS-PAGE reducing

conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 11.67 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below.

MG S S H H H H H H S S G LV P R G S H M A S M T G G Q Q MG R G S E F -AP VA N E LR C Q C L Q T VA GI H F K N I Q S L

KVMPPGPHCT QTEVIATLKN GREACLDPEA PMVQKIVQKM LKGVPK

[ REFERENCES ]

1.Huang S., et.al. (1992) Biochem. Biophys. Res. Commun. 184:922-929.

2.Huang S., et.al. (1992) Am. J. Pathol. 141:981-988.

3.Hanzawa H., et.al. (1994) FEBS Lett. 354:207-212.

4.Hanzawa H., et.al. (1997) J. Biochem. 121:835-841.

5.Hanzawa H., et.al. (1998) J. Biochem. 123:62-70.

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