YB90087Si01

Monocyte Chemotactic Protein 1 (MCP1)

Organism: Rhesus monkey (Simian)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

3th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Monocyte Chemotactic Protein 1

Gene Names: MCP1, CCL2, SCYA2

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Binds to CCR2 and CCR4.

Subcellular Location: Secreted

[ PROPERTIES ]

Residues: Gln24~Pro99 (Accession # P61275), with a N-terminal His-tag.

Grade & Purity: >97%, 10.2kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 10.2 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-QPDAINA  PVTCCYNFTN RKISVQRLAS YRRITSSKCP KEAVIFKTIV  AKEICADPKQ

KWVQDSMDHL DKQIQTPKP

[ REFERENCES ]

1.Timmers H.T.M, et.al .(1990). J .Nucleic Acids Res. 18:23-34.

2.Yoshimura T, et.al.(1991). J .Biochem. Biophys. Res. Commun. 174:504-509.

3.Manczak M, et.al.(2002). J . Autoimmun. 18:259-270.

4.He W, et.al.(2003). J .Infect. Immun. 71:930-936.

5.Chiu B.C, ET.AL.(2003). J. Respir. Cell Mol. Biol. 29:106-116.

6.Pae C.U, et.al.(2004). J .Neuropsychobiology 49:111-114.

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