YB93315Ra01

Calmodulin 1 (CALM1)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

3th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Calmodulin 1

Gene Names: CALM1

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+, AND MASS SPECTROMETRY. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases.

Subcellular Location: Cytoplasm; cytoskeleton; spindle.

[ PROPERTIES ]

Residues: Met1~Lys149 (Accession # P62161), with a N-terminal His-tag.

Grade & Purity: >97%, 18.08 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 18.08 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG

NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE

EVDEMIREAD IDGDGQVNYE EFVQMMTAK

[ REFERENCES ]

1.Giaccone G., et al.  (2005) Ann Onc. 16: 538-48.

2.Schlessinger J., et al. (2000) Cell. 103: 211-25.

3.Yarden Y., et al. (2001) Nat Rev Mol Cell Biol. 2: 127-37.

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