The possible reasons that the actual band size differs from the predicted are as follows:

YBC262Ra01 100µg

Recombinant Tight Junction Protein 1 (TJP1)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Gly23~Asp502

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: F1M4A0

Host: E. coli

Subcellular Location: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Cell junction, tight junction, gap junction.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 56.4kDa

Accurate Molecular Mass: 70kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2.Relative charge: The composition of amino acids may affects the charge of the protein.

3.Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4.Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5.Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

GIAISGGR DNPHFQSGET SIVISDVLKG GPAEGQLQEN DRVAMVNGVS MDNVEHAFAV

QQLRKSGKNA KITIRRKKKV QIPVSHPDPD PVSDNEDDSY DEDVHDPRSG RGALANRRGE

KSWARDRSAS RDRSLSPRSD RRSVASSQPA KPTKVTLVKS RKNEEYGLRL ASHIFVKEIS

QDSLAARDGN IQEGDVVLKI NGTVTENMSL TDAKTLIERS KGKLKMVVQR DERATLLNVP

DLSDSIHSAN ASERDDISEI QSLASDHSVR SHDRPPRRSQ SRSPDQRSEP SDHSTQSPQQ

PSNGSLRSRE EERMSKPGAV STPVKHVDDH TPKAVEEVTV EKHEKQTPTL PEPKPVYAQV

GQPDVDLPVS PSDGVLPNST HEDGILRPSM KLVKFRKGDS VGLRLAGGND VGIFVAGVLE

DSPAAKEGLE EGDQILRVNN VDFTNIIREE AVLFLLDLPK GEEVTILAQK KKDVYRRIVE SD

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