YBA741Ra01 50µg

Recombinant Dickkopf Related Protein 1 (DKK1)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Val36~Asn260

Tags: Two N-terminal Tags, His-tag and GST- tag Accession: D3Z9J1

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in

20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.5 Predicted Molecular Mass: 56.3kDa Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

TEL:4006-871-227 Web:www.ybio.net Email:shybio@126.com

[ USAGE ]

Reconstitute in ddH2O.

TEL:4006-871-227 Web:www.ybio.net

Email:shybio@126.com

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VLINS NAIKNLPPPL GGAGGQPGSA VSVAPGVLYE GGNKYQTLDN YQPYPCAEDE

E C G T D E Y C S S P S R G A A G V G G V Q I C L A C R K R R K R C M R H A M C C P G N Y C K N G I

CMPSDHSHLP RGEIEEGIIE NLGNDHGAGD GYPRRTTLTS KIYHTKGQEG SVCLRSSDCA

TGLCCARHFW SKICKPVLKE GQVCTKHRRK GSHGLEIFQR CYCGEGLACR IQKDHHQTSN

[ REFERENCES ]

1.Florea L., et al. (2005) Genome Res. 15:54-66.

2.Niehrs C. (2006) Oncogene 25:7469-7481.

3.Lin C.L., et al. (2010) J. Am. Soc. Nephrol. 21:124-135.

4.Weng L.H., et al. (2010) Arthritis Rheum. 62:1393-1402.

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